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Title: Extracellular-Ca2+-Induced Decrease in Small Molecule Electrotransfer Efficiency: Comparison between Microsecond and Nanosecond Electric Pulses
Authors: Navickait, Diana
Ruzgys, Paulius
Novickij, Vitalij
Jakutavičiūtė, Milda
Maciulevičius, Martynas
Sincevičiūtė, Rūta
Šatkauskas, Saulius
Keywords: calcium electroporation
calcium
microsecond electroporation
nanosecond electroporation
membrane repair
pore resealing
Issue Date: 2020
Publisher: MDPI
Citation: Navickaite, D.; Ruzgys, P.; Novickij, V.; Jakutaviciute, M.; Maciulevicius, M.; Sinceviciute, R.; Satkauskas, S. Extracellular-Ca2+-Induced Decrease in Small Molecule Electrotransfer Efficiency: Comparison between Microsecond and Nanosecond Electric Pulses. Pharmaceutics 2020, 12, 422.
Series/Report no.: 12;5
Abstract: Electroporation—a transient electric-field-induced increase in cell membrane permeability—can be used to facilitate the delivery of anticancer drugs for antitumour electrochemotherapy. In recent years, Ca2+ electroporation has emerged as an alternative modality to electrochemotherapy. The antitumor effect of calcium electroporation is achieved as a result of the introduction of supraphysiological calcium doses. However, calcium is also known to play a key role in membrane resealing, potentially altering the pore dynamics and molecular delivery during electroporation. To elucidate the role of calcium for the electrotransfer of small charged molecule into cell we have performed experiments using nano- and micro-second electric pulses. The results demonstrate that extracellular calcium ions inhibit the electrotransfer of small charged molecules. Experiments revealed that this effect is related to an increased rate of membrane resealing. We also employed mathematical modelling methods in order to explain the differences between the CaCl2 effects after the application of nano- and micro-second duration electric pulses. Simulation showed that these differences occur due to the changes in transmembrane voltage generation in response to the increase in specific conductivity when CaCl2 concentration is increased.
Description: This article belongs to the Section Drug Delivery and Controlled Release
URI: http://dspace.vgtu.lt/handle/1/4024
ISSN: 1999-4923
Appears in Collections:Moksliniai straipsniai / Research articles

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