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Please use this identifier to cite or link to this item: http://dspace.vgtu.lt/handle/1/4121

Title: Compact Square-Wave Pulse Electroporator with Controlled Electroporation Efficiency and Cell Viability
Authors: Stankevič, Voitech
Šimonis, Povilas
Žurauskienė, Nerija
Stirkė, Arūnas
Dervinis, Aldas
Bleizgys, Vytautas
Keršulis, Skirmantas
Balevičius, Saulius
Keywords: pulsed electric field generator
power electronics
square-wave electrical pulses
electroporation
yeast cells
Issue Date: 2020
Publisher: MDPI
Citation: Stankevic, V.; Simonis, P.; Zurauskiene, N.; Stirke, A.; Dervinis, A.; Bleizgys, V.; Kersulis, S.; Balevicius, S. Compact Square-Wave Pulse Electroporator with Controlled Electroporation Efficiency and Cell Viability. Symmetry 2020, 12, 412.
Series/Report no.: 12;3
Abstract: The design and development of a compact square-wave pulse generator for the electroporation of biological cells is presented. This electroporator can generate square-wave pulses with durations from 3 μs up to 10 ms, voltage amplitudes up to 3500 V, and currents up to 250 A. The quantity of the accumulated energy is optimized by means of a variable capacitor bank. The pulse forming unit design uses a crowbar circuit, which gives better control of the pulse form and its duration, independent of the load impedance. In such cases, the square-wave pulse form ensures better control of electroporation efficiency by choosing parameters determined in advance. The device has an integrated graphic LCD screen and measurement modules for the visualization of the current pulse, allowing for express control of the electroporation quality and does not require an external oscilloscope for current pulse recording. This electroporator was tested on suspensions of Saccharomyces cerevisiae yeast cells, during which, it was demonstrated that the application of such square-wave pulses ensured better control of the electroporation efficiency and cell viability after treatment using the pulsed electric field (PEF).
Description: This article belongs to the Special Issue Information Technologies and Electronics
URI: http://dspace.vgtu.lt/handle/1/4121
ISSN: 2073-8994
Appears in Collections:Moksliniai straipsniai / Research articles

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